Submerged culture of Grifola gargal and G. sordulenta as source of mycelium with antioxidant activity

POSTEMSKY P.D.; CURVETTO N.R.

INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS

BEGELL HOUSE INC

Año: 2014

1521-9437

Submerged culture is an alternative mycelium source for Grifola gargal and G. sordulenta, two rare edible mushrooms related to G. frondosa. This work deals with the study of their mycelia as source of antioxidants. The efficient concentrations of methanolic extracts in both radical scavenging (RS) and reducing power (RP) abilities in G. gargal and in G. sordulenta showed a high antioxidant activity. At the used experimental design, the antioxidant activity mainly depended on the culture conditions rather than on the media composition. Irrespective of the basal culture medium, mycelium methanolic extracts of G. sordulenta obtained from culture in Erlenmeyers showed RS-EQAA and RP-EQAA contents higher than the corresponding values obtained with jar cultures. Under stationary cultivation, G. sordulenta produced ca. 50% higher content in both RS-EQAA and RP-EQAA than the medicinal mushroom G. frondosa. Phenolics correlated with RS-EQAA and RP-EQAA in G. gargal and with RP-EQAA in G. sordulenta; besides, TLC showed these compounds to be at least in part related to the RS capacity. It is concluded that G. gargal and G. sordulenta mycelia are excellent sources of antioxidant metabolites.