Renal dopaminergic system dysfunction and its association with arterial hypertension and renal inflammation in an experimental model of metabolic syndrome induced by fructose overload

NATALIA L RUKAVINA MIKUSIC; KOUYOUMDZIAN, N.M.; MARÍA CECILIA KRAVETZ; ROUVIER E; ROBBESAUL G; ALVAREZ PRIMO M; DEL MAURO J; LEE HJ; PUYO AM; JORGE E TOBLLI; BELISARIO E. FERNÁNDEZ; CHOI, M.R.

Congreso; Congreso de Sociedad Argentina de Investigación Clínica (SAIC) ? Sociedad Argentina de Inmunología (SAI) ? Sociedad Argentina de Farmacología Experimental (SAFE); 2016

Introduction: Renal dopaminergic system (RDS) promotes sodium excretion and anti-inflammatory actions. A high fructose diet induces metabolic and hemodynamic changes that could be associated with an impairment of the RDS, leading to renal inflammation, sodium retention and arterial hypertension. Aim: to evaluate RDS state and its association with the development of hypertension and overexpression of renal inflammatory markers in fructose overloaded (FO) rats. Methods: Male Sprague Dawley rats were assigned to Control (C, tap water) or FO (10% w/v of fructose solution) groups, during 4, 8 and 12 weeks (n=8/group/period). Urinary L-dopa and dopamine (DA), diuresis and albuminuria were determined. Systolic blood pressure (SBP) and metabolic parameters were measured. Western blot analysis of renal expression of D1R, NFκβ, IL-6, TNF-α, TGF-β1 and nephrin were performed. Results: FO increased SBP (mmHg, C4: 121±8 vs. F4: 145±1*; C8: 130±4 vs. F8: 161±10#; C12: 133±5 vs. F12: 163±4#), which positively correlated (R2=0.78; p