Evaluation of a low dose of Angiotensin II infusion as regulator of renal dopaminergic system through organic cation transporters

KOUYOUMDZIAN, N.M.; NATALIA L RUKAVINA MIKUSIC; ROBBESAUL G; ALVAREZ PRIMO M; SUSANA GORZALCZANIC; MARCELA PANDOLFO; GIRONACCI, M.M.; ROSON MI; JORGE E TOBLLI; BELISARIO E. FERNÁNDEZ; CHOI, M.R.

Buenos Aires

Congreso; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2017

Sociedad Argentina de Investigación Clínica (SAIC)

The aim of this study was to evaluate the effects of a low dose of angiotensin II(ANG II: 1 ug/kg/hour) on renal dopaminergis system (RDS) through determinationof the organic cation transporters (OCTs and OCTNs) expression in renal cortex,dopamine (DA) urinary levels, Na+,K+?ATPasa (NKA) activity and renal function.Under inhibition of DA synthesis by benserazide, anesthetized male SpragueDawley rats were infused with isotonic saline solution during 120 minutes andrandomized in nine different groups: control, pargyline plus tolcapone (P+T),ANG II, DA, ANG II+DA, D-22, ANG II+D-22, DA+D-22 and ANG II+DA+D-22. Renalfunctional parameters and direct blood pressure were determined and urinary DAconcentration was quantified by HPLC. Expression of OCTs, OCTNs, D1-subtypereceptor (D1R) and NKA in membrane preparations from renal cortex tissues weredetermined by Western Blot. NKA activity was determined using in vitro enzymeassay. Compared to P+T group, DA infusion significantly increased fractionalexcretion of sodium (FENa) and DA excretion (*: p