Renal dopaminergic system dysfunction associated to high blood pressure and inflammation in fructose overload induced metabolic syndrome

PUYO AM; NATALIA L RUKAVINA MIKUSIC; KOUYOUMDZIAN, N.M.; ROBBESAUL G; ALVAREZ PRIMO M; LEE HJ; TOBLLI, J.E.; BELISARIO E. FERNÁNDEZ; CHOI, M.R.

Milan

Congreso; 27th European Meeting on Hypertension and Cardiovascular Protection; 2017

European Society of Hypertension

Introduction: The renal dopaminergic system (RDS) promotes sodium excretion and anti-inflammatory actions. Fructose overload in the rat is an experimental model of metabolic syndrome that induces hemodynamic and metabolic changes associated to an impairment of the RDS, leading to renal inflammation, sodium retention and blood pressure elevation. Aim: to evaluate RDS state and its relation to hypertension and expression of renal inflammatory markers in fructose overloaded (FO) rats. Methods: Two groups of male Sprague Dawley rats of six weeks at the beginning of the treatment were studied: Control (C, tap water to drink) or FO (10% w/v of fructose solution to drink), during 4, 8 and 12 weeks (n=8/group/period). Urinary L-dopa and dopamine (DA) (HPLC), diuresis and albuminuria were determined. Systolic blood pressure (SBP) and metabolic parameters were measured. Western blot analysis of renal expression of D1R, NFκβ, IL-6, TNF-α, TGF-β1 and nephrin were performed. Results: FO increased SBP (mmHg, C4: 121±8 vs. F4: 145±1*; C8: 130±4 vs. F8: 161±10#; C12: 133±5 vs. F12: 163±4#), which positively correlated (R2=0.78; p