MULTIPLE MYB TRANSCRIPTIONAL FACTORS ARE INVOLVED INTO CONDENSED TANNIN BIOSYNTHESIS REGULATION IN LOTUS SPP.

ESCARAY F.J.; PEREA GARCÍA A.; MARCO A.; CARRASCO P.; PAOLOCCI F; RUIZ O.A..

CORDOBA

Congreso; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Condensed tannins (CTs) biosynthesis in plants has been shown to be transcriptionally regulated through the MBW complex. This complex is formed by the physical interaction of three transcription factors (TFs) belonging to the R2R3-MYB, bHLH, and WDR protein families. It appears to be redundancy for the bHLH cofactors, while the MYB protein generally provides specificity. In L. japonicus, three MYBs involved in to CT regulation (TT2a, TT2b and TT2c) have been described. However, our transcriptional studies performed on crop species with polymorphism for this trait (L. tenuis (Lt) and L. corniculatus(Lc)) suggest that almost another six MYB proteins could be form part of different MWB complex related to CT biosynthesis regulation.Whole transcriptome sequencing analysis (Illumina Hi-seq 1500) was performed fromLt and Lc shoots samples and transcripts were obtained with de novo assembly methodology. Orthologue sequences from several MYB TFs involved in TC biosynthesis regulation from different species were obtained.We selected nine MYB TFs related to TT2, MYB14, MYBPA and MYB5 genes. The relative expression of MYBs candidates (qRT-PCR) was measured in Lt, Lc and Lt x Lc genotypes using specific primers.The gene expression and its correlation with CT levels suggest that four of them (TT2b, TT2d, MYBPA2 and MYB5) are the best candidates.