Antibodies reacting with neighbouring gangliosides are present in rabbits with ganglioside induced experimental neuropathy

MOYANO AL; COMÍN R; VILCAES AA; FUNES SC; ROTH GA; IRAZOQUI FJ; NORES GA

GLYCOBIOLOGY

OXFORD UNIV PRESS INC

Lugar: Oxford; Año: 2012 p. 1768 - 1774

0959-6658

Immunization of rabbits with bovine brain gangliosidesinduced an experimental neuropathy, with clinical signsresembling Guillain?Barré syndrome. All the immunizedanimals developed immunoglobulin G immunoreactivityto GM1 ganglioside. In a few (4 of 27) animals, an additionalanti-ganglioside antibody population showing anunusual binding behavior was detected. Enzyme-linkedimmunosorbent assay and thin-layer chromatographyimmunostaining analyses showed that the binding of theseunusual antibodies required the presence of two co-localizedgangliosides. Maximal interaction was observed to amixture of GM1 and GD1b, but the antibodies also showed?density-dependent? binding to GD1b. The antibodies werepurified by affinity chromatography and displayed theability to target antigens in biological membranes (ratsynaptosomes)