Determination of nitrotyrosine in stressed rats placenta.

M.C ROMANINI; A. ROLANDO; M.C ROJO; L. TARDITI; C.A SOÑEZ; M.T MUGNAINI; L. PASTORINO; A. BOZZO; H.F GAUNA

Tafí del Valle, Tucumán

Congreso; XXIII Jornadas científicas de la Asociación de biología de Tucumán.; 2006

Asociación de Biología de Tucumán

Maternal stress produces a placentary oxidative profile alteration. Peroxynitrites are generated when nitric oxide (NO) reacts with reactive oxygen species. Peroxynitrite can affect signal traduction by nitrating aromatic amino acids such as tyrosine of the cellular membrane proteins forming nitrotyrosine residues in the placentary tissue. This would be a marker of the tissue damage by the action of the reactive oxygen species. Our aim was to determine the presence of nitrotyrosine in the placentary tissue of stressed pregnant rats as an oxidative stress marker. Wistar rat placentas of 12 and 17days pregnancy were used as controls © and they were chronically stressed (E). Immunostaining of nitrotyrosine residues in placentary tissue was done whit rabbit anti-nitrotyrosine polyclonal antibody 1/500. The nitrotyrosine was evident in the endotelial blood vessels of the placentary laberinth at the 17 th pregnancy day. No positive marks were observed at the 12 th pregnancy day. This result is probably to the little development of the placentary laberinth. Together whit the development of the placentary laberinth zone angigenesis and vasculogenesis process happen wich be ischemia-reperfusion produce an increase in the reactive oxygen species. The oxidative impact in the placentary tissue is evident in the last days of gestation in chronically IMO stressed rats.