Biodiversity of Saccharomyces cerevisiae strains in different parts of the vineyard ecosystem

GONZÁLEZ M.R.; STURM M.E.; CIKLIC I.; COMBINA M.; MERCADO L.

Recife

Jornada; V Jornadas Latinoamericanas de Biología y Biotecnología de Levaduras; 2015

Red Latinoamericana de Levaduras

Grapes and wine productions are activities with great impact in Argentina economy. The development of them through adding value and diversification of the production can be achieved by a microbiology approach. The knowledge and understanding of phenomena intervening in the presence of different of Saccharomyces cerevisiae strains in the vineyards has been attempt in wine regions all over the world. In Argentina, Malbec is the main grape variety produced and the ?Zona Alta del Río Mendoza? (ZARM) represents the principal area of its cultivation. In the present study the persistence of S. cerevisiae in a Malbec vineyard from ZARM wine region was evaluated, with the aim of study the reservoirs of these yeasts through the phenological grape cycle and to contribute to maintain the biodiversity of the region. S. cerevisiae populations in different stages from harvest to flowering were evaluated by collecting samples of grapes, soil, bark, buds and flowers. S. cerevisiae strains were molecular differentiated by interdelta PCR and molecular patterns obtained were compared to determinate biodiversity in each stage and sample and to evaluate persistence across the time in the lapse evaluated. Different S.cerevisiae strains populations from the different niches of the vineyard ecosystem were verified. A dynamic behavior, through the different phases of the viticole cycle was observed. Moreover, the S.cerevisiae strains isolated in each stage came from only one type of sample. This work contributes to the preservation of the genetic resources of regional interest and the results obtained allow a better knowledge of the biodiversity of native vineyard yeasts, allowing the future development of inoculums contributing to the improvement of Argentinean wines and a better expression of ?terroir? in Malbec wines from Mendoza.