Yeasts isolated of bloodstream infections and catheter colonization in hospitalized pediatric patients

GIUSIANO G; MANGIATERRA M; ROJAS F; GÓMEZ V; DÍAZ MC

San Antonio, Texas.

Congreso; The 15th Congress of the International Society for Human and Animal Mycology. (ISHAM); 2003

International Society for Human and Animal Mycology. (ISHAM)

Funguemia is a growing problem in hospitalized chills, particularly in neonatal and intensive care units patients (UTI). This study was performed between February 1999-2002 in a Hospital Pediátrico ?Juan Pablo II?, Corrientes (Argentina). The aim was to determine: a) The incidence of funguemias and yeast catheter colonization. b) The frequency and antifungal susceptibility profile of species isolated. By lisis centrifugation 9.465 bloodstream were studied and 759 catheters by Maki and Cleri methods. Kreger van-Rij method and Api ID 32C (bioMérieux) were used for identification. The antifungal sensitivity test employed was the NCCLS micromethod M27A. Following Streptoccocus pneumonia (23%) and Staphylococcus aureus (21,4%) the yeast was de 3rd more prevalent cause of bloodstream infection (11,72%). Yeast was the most frequent catheter colonization (32%). Of the 88 funguemia 31 were from neonates in intensive care units, 42 from patients in UTI and 15 from oncologics. Of the 64 yeast catheters colonization, 20 were from neonates, 37 were from UTI and 7 from oncologics. From neonates Candida albicans (C.albicans) (36%) and C. parapsilosis (36%) were the most frequents species isolated in bloodstream. In catheters C. parapsilosis (50%). Fron oncológics C. parapsilosis was the predominant specie (55,4%). Bloodstreams in UTI showed C. albicans (44,4%) and C. tropicalis (36,1%). Catheters C. albicans (45,9%). Trichosporon cutaneum, C. krusei, C. glabrata and others Candida spp. were isolated from blood and Malassezia species from catheters. These species are considered emergent nosocomial pathogens because of they increasingly incidence and antifungal resistance or potential for developing. All the isolates were susceptible to amphotericin B. C. albicans (6,7%), C. tropicalis (8,3%) and C. glabrata (50%) were resistant to fluconazole. C. krusei (100%) were resistant to fluconazole and itraconazole. These data showed the emergency of others yeast than C. albicans as cause of funguemia and catheter colonization. Continued surveillance will be important to detect emergence of resistance to azoles.