Triazolic antifungal activity against Malassezia strains

CARRILLO MUÑOZ AJ; GIUSIANO G; QUINDOS G; ROJAS F; SOSA MA; ERASO E

Torino

Congreso; 3ª Trends in Medical Mycology; 2006

European Confederation of Medical Mycology (ECMM) and EORTC Infectious Diseases Group

Antifungal susceptibility profile of 72 isolates of different Malassezia species to itraconazole (ITZ), fluconazole (FZC), voriconazole (VRZ), posaconazole (PSZ), albaconazole (ABZ) and ravuconazole (RVZ) were studied. Sixteen Malassezia furfur (M. furfur), 26 M. globosa, 14 M. sympodialis, 5 M. pachydermatis, 5 M. slooffiae, 1 M. restricta, 5 Malassezia spp. strains were tested. Microdilution method based in CLSI M27-A2 reference method with the ox-bile aggregate was used. Microplates were incubated 5 days to 32ºC. Range of concentration used were 0,01 ? 16 μg/ml, except for FCZ: 0,125-256 μg/ml. Minimal inhibitory concentration (MIC) was defined as the concentrations of antifungal agent that were able to inhibit 50% of the isolates. Quality control strains were Candida parapsilosis ATCC 22019, Candida krusei ATCC 6258, Malassezia furfur CBS 7019 y Malassezia sympodialis CBS 7222 were included each time isolated were tested. High in vitro antifungal activity for ITZ, VRZ, PSZ, ABZ and RVZ against all Malassezia strains studied was observed. FCZ showed lower activity than the others antifungal agents tested, moreover variations in its activity between Malassezia species were observed. M. sympodialis and M. pachydermatys were more susceptible to FCZ. M. furfur and M. restricta were the species less susceptible to this antifungal agent.