OPTIMIZATION OF A SYNTHETIC MEDIUM FOR CELLULASES SECRETION OF Trichoderma harzianum NATIVE OF MISIONES

CASTRILLO, M. L.; MARTÍNEZ, C. N.; RODRÍGUEZ, M. D.; BICH, G. A.; ZAPATA, P. D.; VILLALBA, L. L.

Mar del Plata

Congreso; Reunión SAIB 2015; 2015

A reduction in the cost of bioethanol production can be achieved by reducing the cost of raw materials or cellulaseenzymes. The aim was to optimize a synthetic culture medium to maximize the cellulases secretion of a Trichodermaharzianum strain native of Misiones. A Box-Behnken response surface design was performed to analysecarboxymethylcellulose (CMC) as sole carbon source; and urea, yeast extract and ammonium sulfate as nitrogensources. The effects of the factor were tested at three levels and 29 tests were conducted, including a central pointquintupled. The synergistic effect of the three cellulases was determined by the filter paper method (FPU). The EGs and CBHs activities were determined by the method of DNS acid, and BGLs activity was determined by the methodof p-nitrophenyl-B-glucoside. Taking the days with the highest enzyme activity (without statistically significantdifferences), a positive influence (statistically significant) of CMC was observed in all the analysed enzymes. Also, itwas observed a statistically significant and positive interaction between urea and yeast extract regarding EGs activity;and a statistically significant and positive influence of urea, as well as its interaction with CMC for FPU activity. Itwas determined which combination of factors levels led to the optimization of cellulasess secretion of a T. harzianum native of Misiones.