Wood flour as carbon source for the enzymatic secretion of white rot fungi

RODRÍGUEZ, M. D.; BARCHUK, L.; CASTRILLO, M. L.; VELÁZQUEZ, J. E; KRAMER, G. R.; ZAPATA, P. D.; VILLALBA, L. L.

Porto Alegre

Simposio; 8 Latin American Biodeterioration and Biodegradation Symposium; 2013

American Biodeterioration and Biodegradation Symposium

Cellulose is the main component of plant cell wall, the most abundant polymer on the earth and a major renewable resource. Rot fungi, for their enzymatic capacity, are excellence organisms capable of degrading lignocellulosic material. The cellulase enzyme system consists of three main enzymes which act synergistically to degrade cellulose to glucose. One of these are endoglucanase, which acts on reducing ends of cellulose. Therefore, the objective was to assess the endoglucanases secretory capacity by white rot fungi native of Misiones (Argentina). Cellulolytic power was tested in 13 strains, using pine and eucalyptus flour as carbon source. The strains were inoculated for duplicate in Mandels medium with carbon source at 1% W/V and 80rpm reciprocal shaking at 29°C, taking aliquots every 72 hours for 15 days. Enzymatic determination was performed by IUPAC technique on each aliquot, finding increased secretion from the 144hs of incubation. Elevated values endoglucanase activity were found using native strains, which are promising for cellulase production, and on the other hand, wood flour will function as a accessible carbon source for the fungus and economical in terms of productivity.