Refinement of the technique of drug administration through the application of electrospun transdermal membranes

ECHARTE, STELLA MARIS; ORALLO, DALILA E. ; CÁMARA, CAROLINA; LA COLLA, ANABELLA; RIVERO GUADALUPE; CHISARI, ANDREA

Mar del Plata

Otro; Reunion Anual de Sociedades de Biociencias - SAIC-SAFIS; 2022

The controlled release of bioactive agents can be achieved from their encapsulation in a porous matrix in a controlled or sustained manner over time, generally by diffusion.The use of electrospun cellulose acetate membranes as transdermal patches with vitamins E has been reported, and their in-vitro release has been characterized. However, no in vivo studies have been carried out. Polymeric controlled release systems allow evaluating different doses and modulating their release over time, through the alteration of the geometry, architecture, size, surface properties and composition of the membranes. We aimed to prepare and characterization of nanofibrous matrices electrospun cells loaded with vitamin E for invivo use as transdermal patches in wound healing in a Wistar rat model.Polymeric solutions of poly(ε-caprolactone) (PCL, 80000Da) at 15-20% w/v were prepared. Different concentrations of Vitamin E were incorporated 1% (A); 6% (B) and 12% (C).The punch technique was used to generate the wound in the middle dorsal part of the rat. A membrane disk was placed directly over the area to be healed.The efficient incorporation of vitamin E into the membranes was obtained by Agilent 8453 UV-visible absorption spectroscopy, performing a calibration curve with Vitamin E as standard compound at 296 nm. Morphological characterization was performed by scanning electron microscopy (SEM). Regarding the in vivo model, the diameter of healing over time was measured and histological sections of skin were made and stained with the H&E technique.The following results of mg Vit E/g membrane were obtained for (A) 4.90±0.02; (B) 16.86±0.05 and (C) 46.05±0.03. We observed that the diameter of the wound decreases faster in the membranes containing (B) and (C). Regarding histology greater amount of scar tissue is associated higher concentration of vitamin.In conclusion, improvements in the healing process are observed, which demonstrates the effectiveness of the membranes in drug dosing.