The production of nitric oxide in the coeliac ganglion modulates the effect of cholinergic neurotransmission on the rat ovary during the preovulatory period

DELSOUC, MB; DELLA-VEDOVA, MC; RAMIREZ, DARIO; DELGADO, SM; CASAI, M

NITRIC OXIDE-BIOLOGY AND CHEMISTRY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: AMSTERDAM; Año: 2018

1089-8603

MARIA BELEN DELSOUC, María Cecilia Della Vedova, Darío Ramírez, SilviaMarcela Delgado, Marilina CasaisThe aim of the present work was to investigate whether the nitric oxide produced by the nitric oxide/nitric oxidesynthase (NO/NOS) system present in the coeliac ganglion modulates the effects of cholinergic innervation onoxidative status, steroidogenesis and apoptotic mechanisms that take place in the rat ovary during the first proestrous.An ex vivo Coeliac Ganglion- Superior Ovarian Nerve- Ovary (CG-SON-O) system was used. Cholinergic stimulationof the CG was achieved by 10-6 M Acetylcholine (Ach). Furthermore, 400 μM Aminoguanidine (AG) ? an inhibitor ofinducible-NOS was added in the CG compartment in absence and presence of Ach. It was found that Ach in the CGcompartment promotes apoptosis in ovarian tissue, probably due to the oxidative stress generated. AG in the CGcompartment decreases the release of NO and progesterone, and increases the release of estradiol from the ovary.The CG co-treatment with Ach and AG counteracts the effects of the ganglionic cholinergic agonist on ovarianoxidative stress, increases hormone production and decreases Fas mRNA expression. These results suggest that NOis an endogenous modulator of cholinergic neurotransmission in CG, with implication in ovarian steroidogenesis andthe apoptotic mechanisms that take place in the ovary during the preovulatory period in rats.