A soybean-based diet protects against cadmium-induced apoptosis in the aorta of rat

PEREZ DIAZ, M; PLATEO-PIGNATARI, MG; FILIPPA, V; MOHAMED, F; EDUARDO MARCHEVSKY; GIMENEZ, MARIA SOFIA; DARIO C. RAMIREZ

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY

ELSEVIER GMBH

Año: 2019

0946-672X

Cadmium (Cd) exposure has been associated to an increased risk of cardiovascular diseases. The diet is a modifiablesource of protecting or damaging factors that may affect this risk. Herein we hypothesized that a soybean-based diet(SBD) can modulate Cd-induced pro-inflammatory and pro-apoptotic profile, and thus protect the vascular wall of aortain a rat model. To test our hypothesis, we fed male Wistar rats for 60 days with a casein-based diet (CBD) or a SBD.These animals were also exposed to water without (CBD-Co/SBD-Co) or with 15(CBD-15Cd/SBD-15Cd) or 100(CBD-100Cd/SBD-100Cd) ppm of Cd. Inflammatory parameters (mRNAs or proteins) were measured in thoracic aortatissue. These included inducible (iNOS) and endothelial (eNOS) nitric oxide synthases, cyclooxygenase-2,intracellular-adhesion molecule-1 and vascular cell-adhesion molecule-1. As pro-apoptotic parameters we measuredBax and Bcl-2 mRNA/protein, as well as TUNEL positive cells in the aorta tissue. Compared to CBD-Co, Cdinflammatory and apoptosis markers increased in aorta, and these were CBD-15Cd