Oxidative stress and altered steroidogenesis in the ovary by cholinergic stimulation of coeliac ganglion in the first proestrous in rats. Implication of nitric oxide

DELSOUC, MB; DELLA-VEDOVA, MC; DARIO C. RAMIREZ; ANZULOVICH, A.C.; DELGADO, SM; CASAI, M

NITRIC OXIDE-BIOLOGY AND CHEMISTRY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2016 vol. 53 p. 45 - 53

1089-8603

An ex-vivo Coeliac Ganglion-Superior Ovarian Nerve-Ovary (CGeSONeO) system from virgin rats in thefirst proestrous was used to test whether cholinergic stimulation of CG affects oxidative status andsteroidogenesis in the ovary. The CG and the O were placed in separate buffered-compartments, connectedby the SON, and the CG was stimulated by acetylcholine (Ach). To test a possible role of nitricoxide (NO) in the ovarian response to cholinergic stimulation of CG, aminoguanidine (AG) e an inhibitorof inducible-NO synthase was added to the O compartment. After 180 min incubation, the oxidativestatus was assessed in O whereas nitrite and steroidogenesis were assessed at 30, 120 and 180 min. Achin CG decreased the total antioxidant capacity, but increased NO production and protein carbonization inO. Ach stimulation of CG increased estradiol, but decreased progesterone release in O by reducing themRNAs related to their synthesis and degradation. The addition of AG to the O compartment caused anopposite effect, which was more pronounced in the presence of Ach in the CG compartment than in itsabsence. These results show that the stimulation of the extrinsic-cholinergic innervation of the O increasesthe concentration of NO, causes oxidative stress and modulates steroidogenesis in the first ratproestrous.