Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates

FLORENCIA ELIZABETH PICCINNI; DIEGO HERMAN SAUKA; SILVINA GHIO; MARÍA PIA VALACCO; MÁXIMO LISANDRO RIVAROLA; ORNELLA MAILÉN ONTAÑON; FLORENCIA ELIZABETH PICCINNI; PAOLA MÓNICA TALIA; DIEGO HERMAN SAUKA; ELEONORA CAMPOS; MARÍA PIA VALACCO; ORNELLA MAILÉN ONTAÑON; PAOLA MÓNICA TALIA; ELEONORA CAMPOS; SILVINA GHIO; MÁXIMO LISANDRO RIVAROLA

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2019 vol. 126 p. 811 - 825

1364-5072

AIMS: Lignocellulosic biomass deconstruction is a bottleneck for obtainingbiofuels and value-added products. Our main goal was to characterize thesecretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost-efficient enzymatic cocktails.METHODS AND RESULTS: We identified 205 potential CAZymes in the genome ofCellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretomeanalysis of supernatants from cultures in either extruded wheat straw (EWS),grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identifiedwhich proteins played a role in lignocellulose deconstruction. Growth on CMCresulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10xylanases, while growth on SCR or EWS resulted in the identification of adiversity of CAZymes. From the 32 GHs predicted to be secreted, 22 wereidentified in supernatants from EWS and/or SCR cultures, including endo- andexoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β-xylosidase, a β-glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10.CONCLUSIONS: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes.SIGNIFICANCE AND IMPACT OF THE STUDY: Cellulomonas sp. B6 could serve as a source of lignocellulose-degrading enzymes applicable to bioprocessing andbiotechnological industries.