Diet composition of reintroduced Red-and-Green Macaws reflects gradual adaptation to life in the wild

VILACOBA, ELISABET; BERKUNSKY, IGOR; KOPUCHIAN, CECILIA; VOLPE, NOELIA L; BRAUKMANN, THOMAS W A; LIJTMAER, DARÍO A; THALINGER, BETTINA; DI GIACOMO, ADRIÁN S; STEINKE, DIRK

THE CONDOR

COOPER ORNITHOLOGICAL SOC

Año: 2021 vol. 124

0010-5422

Over the last two centuries, the Red-and-Green Macaw (Ara chloropterus) has become locally extinct in Argentina. In anattempt to restore its key ecosystem functions as both disperser and regulator of large-seeded plants, a reintroductionproject was initiated at the Iberá National Park in northeastern Argentina. The ability of released individuals to find foodis crucial, especially when working with captive-bred animals, as long-term establishment of a self-sustaining population depends on their short-term ability to exploit wild food sources. Monitoring of feeding habits is usually conductedthrough behavioral observation, but in recent years DNA metabarcoding has emerged as an alternative for obtaininghighly resolved data on diet composition. In this study, we use a combination of both techniques to characterize thebreadth and composition of the reintroduced macaws´ diet. In addition, we compare the efficiency of both observationaland molecular techniques to assess diet composition in a frugivorous bird. Individuals fed on a variety of plant species(n = 49) belonging to a broad phylogenetic spectrum (28 families). Dietary richness estimated by direct observation andDNA metabarcoding was similar, though smaller than the combination of the two datasets as both techniques detectedat least 15 species not recorded by the other method. While the total number of detected species was higher for observational data, the rate of species-detection per sampling day was higher for DNA metabarcoding. These results suggestthat a combination of both methods is required in order to obtain the most accurate account of the total diversity of thediet of a bird species. The ability of reintroduced macaws to successfully exploit local food resources throughout the yearindicates a good level of adjustment to the release site, an important step towards the creation of a stable, self-sustainingpopulation of Red-and-Green Macaws in Northern Argentina.