Effects of oxidized low-density lipoprotein (OXLDL) on prostatic stromal cells derived from patients with benign prostatic hyperplasia

LÓPEZ SEOANE MANUEL; CUELLO RUBIO MARIANA; QUINTAR AA; ROLDÁN FRANCO; MALDONADO CRISTINA A

Evento virtual

Congreso; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2020

Sociedad Argentina de Investigación Clínica

Benign Prostatic Hyperplasia (BPH) affects elderly men, being theresult of an excessive and uncontrolled cellular proliferative processof both epithelial and stromal prostatic compartments. Novelevidence suggests that dyslipidemias and other factors of the MetabolicSyndrome are associated to BPH progression and aggressiveness.However, little information is available about the pathogenicmechanisms promoting prostatic growth in atherogenic contexts.We therefore aimed to analyze in vitro the effects of OxLDL on primarycell cultures. Prostatic stromal cells, surgically harvested frompatients with BPH (n=3), were isolated, cultured, and stimulated withOxLDL (20 μM or 100 μM, simulating an atherogenic state) or vehiclefor 24h and 48h.OxLDL induced cell proliferation, as assessed by BrdU incorporationand Ki67 immunocytochemistry, mainly at lower concentrations andin a time-dependent manner (p