Effects of Oxidized Low-Density Lipoprotein (OxLDL) on prostatic stromal cells derived from patients with Benign Prostatic Hyperplasia

CUELLO RUBIO MARIANA; MALDONADO CRISTINA; ROLDÁN GALLARDO FRANCO F.; QUINTAR AMADO; LÓPEZ SEOANE MANUEL

Jornada; Reunión Anual de Sociedades de Biociencias 2020; 2021

Reunión Anual de Sociedades de Biociencias

Benign Prostatic Hyperplasia (BPH) affects elderly men, being the result of an excessive and uncontrolled cellular proliferative process of both epithelial and stromal prostatic compartments. Novel evidence suggests that dyslipidemias and other factors of the Metabolic Syndrome are associated to BPH progression and aggressiveness. However, little information is available about the pathogenic mechanisms promoting prostatic growth in atherogenic contexts. We therefore aimed to analyze in vitro the effects of OxLDL on primary cell cultures. Prostatic stromal cells, surgically harvested from patients with BPH (n=3), were isolated, cultured, and stimulated with OxLDL (20 μM or 100 μM, simulating an atherogenic state) or vehicle for 24h and 48h.OxLDL induced cell proliferation, as assessed by BrdU incorporation and Ki67 immunocytochemistry, mainly at lower concentrations and in a time-dependent manner (p