The phtalate DEHP downregulates the pituitary estrogen receptor alpha expression and impact on lactotroph and gonadotroph cell proliferation

SOSA LDV; S. GUTIERREZ; TOLEDO J ; TORRES AI; PEREZ P; DE PAUL A L

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2019

The normal functioning of the pituitarygland is the result of a balanced mechanism capable of rhythmically control the proliferation and hormonal secretion ofdifferent endocrine cell populations in response to endogenousfactors such as Estrogen (E2) acting through estrogen receptors(ER). Also, it has been shown that exogenous agents asphthalates, substances used for plastics manufacture, might actas endocrine disruptors in several tissues, simulating E2 effects.The purpose of this study was to analyze the Di-2-ethylhexylphthalate (DEHP) effect on pituitary ERa expression and itsimpact on cell proliferation. We used Wistar female ratsprenatally treated with DEHP. At 21 days of postnatal lifepituitary glands were extracted and intracellular ERa (ERa) andmembrane ERa (mERa) positive cells were quantified by flowcytometry (FC). Also, primary pituitary cell cultures werestimulated with DEHP, E2 or DEHP+E2 for 72 h. The lactotrophand gonadotroph proliferation was quantified by doubleimmunostaining (ki67+PRL or Ki67+bLH). The percentages ofERa+ and mERa+ cells were quantified by FC. ANOVA Fisher(p