EFFECTS OF TESTOSTERONE ON NEUTROPHIL BEHAVIOR IN ATHEROGENIC CONDITIONS

AMADO QUINTAR; MARÍA VICTORIA SCALERANDI; CRISTINA ALICIA MALDONADO

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2019

Sociedad Argentina de Investigación Clínica

TESTOSTERONE EFFECT ON NEUTROPHIL BEHAVIOR IN ATHEROGENIC CONDITIONSScalerandi MV, Carabajal Miotti C, Gonzalez Silva N, Ortega-Gomez A, Maldonado C, Ruiz de Frattari S, Soehnlein O, Quintar AA Many risk factors have been associated to the development of vascular pathology playing the sex hormones a pivotal modulating role on metabolic disorders and the cardiovascular outcome. In addition to their well-studied role in acute infections, neutrophils have been pointed as contributors of several inflammation-induced injuries, particularly during the onset and progression of atherosclerosis. In spite of the diverse effects of androgens on the immune and cardiovascular systems, there are no reports on the influence of male sexual hormones on neutrophils in atherogenic conditions. We therefore aimed to examine the effect of testosterone on neutrophil behavior in basal and hyperlipidemia milieu. Human neutrophils were isolated from peripheral blood of healthy men (18?65 years old) using Polymorphprep? and stimulated in vitro with testosterone (T, 10-7M) or vehicle in presence and absence of LDL (100 ug/ml). The expression of surface neutrophil activation markers (CD11b, CD11a, CD62L and CXCR2) and the phagocytosis of FITC-labeled latex beads were evaluated by FACS. Neutrophil adherence to endothelial adhesion molecules (P-selectin, ICAM1 and VCAM1) was assessed by functional static adhesion assay. Statistical analysis: Paired t test, p