Diabetic nephropathy in eSS rats and their modulation with polyunsaturated fatty acids (n6 / n3) and the antioxidant nordehydroguaiaretic acid

BERTOZZI J; GARCÍA N; DIAZ-GEREVINI GT; REPOSSI G; LOPEZ CB

Córdoba

Jornada; XX Jornadas de Investigación Científica de la FCM-UNC; 2019

Secretaría de C y T de la Facultad de Ciencias Médicas, UNC

Diabetes is a systemic and chronic disease; among its most important complications is nephropathy. Chronic inflammatory status and increased oxidative stress produce glomerular lesions in relation to the severity of the disease. Proteinuria is increased considering it a marker of kidney damage. There is evidence that polyunsaturated fatty acids (PUFAs) and antioxidants could modulate, at least in part, these processes. The eSS rats spontaneously develop type 2 diabetes, being a valuable experimental model. The aim of the present work was to analyze levels of oxidative stress markers, nflammatory and morphological alterations in kidneys of eSS rats treated with PUFAs and nordehydroguaiaretic acid (NDGA), comparing them with those of eSS rats without treatment and healthy controls.Male rats were used: 4 Wistar (healthy controls) and 20 eSS that were divided into 5 groups (n=4 each), control groups received only the saline solution used as vehicle and the others received intraperitoneally, monthly for 12 months, 6.25mg / kg of PUFAs ?6 or ?3 with / without the addition of 1.9mg / kg of NDGA. Plasmatic metabolic markers (glycaemia, HbA1c), inflammation (reactive prot C, IL-6) and oxidative stress (GGTP) in renal tissue were determined using spectrophotometric techniques. Histopathological study of the kidneys was performed. The data obtained were analyzed by ANOVA and Tukey test, (p?0.05).In the eSS rats significant increases were found in fasting blood glucose (eSS rats ?129 vs 107.8 mg / dl in Wistar), HbA1c (?7.7% eSS vs 5% Wistar) and Reactive C Protein (Control groups eSS and ?6 ?7.6 mg / dl; ?6 + NDGA and ?3 ?4.6 mg / dl; ?3 + NDGA 3.4 mg / dl and Wistar 0.3 mg / dl). IL-6 evels were higher (2.5 to 3.7 times) in kidney than in plasma. The ?3+NDGA treatment reduced the values ??of IL-6, 12 to 72% higher, observed in eSS rats. GGTP activity was higher (52%) in eSS Control than Wistar, the treatments used were able to reduce it. Histological abnormalities were observed in the kidneys of eSS rats.The treatment with PUFAs ?3 and NDGA was the most effective, decreasing the values ??of the systemic and renal parameters analyzed in diabetic eSS rats.