Naringin prevents testicular oxidative stress generated by experimental type 1 Diabetes mellitus

RODRIGUEZ V; DIAZ DE BARBOZA, G; MOINE L; RÍOS DELGADO C; TOLOSA DE TALAMONI N

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Jornada; Jornada de Investigación Científica de la FCM de la UNC; 2018

A high percentage of young men with type 1 Diabetes mellitus (D.m.) havedecreased fertility. Hyperglycemia triggers tissue damage by generatingoxidative stress, the testes being highly sensitive to this condition.  That is why we have evaluated the effect ofnaringin (NAR), a natural antioxidant, on the oxidative stress generated by theexperimental D.m. at the testicular level. Two month old male Wistar rats (n =5 for each experimental group) were used: a) controls; b) STZ: treated with 60mg streptozotocin (STZ) / kg body weight, intraperitoneally; c) STZ + NAR40:STZ rats treated with 40 mg NAR / kg body weight subcutaneously; d) STZ +NAR80: STZ rats treated with 80 mg NAR / kg body weight, subcutaneously. Thecontrol group + NAR was not included because it has been shown that NAR doesnot modify the redox state in sperm tissue of healthy animals. The rats weresacrificed at 30 days post-treatment, and serum levels of glucose and HbA1c,body weight, testicular size and weight were determined. In testis homogenates,the total glutathione (GSH) content and the enzimatic activities of theantioxidant system superoxide dismutase (SOD) and catalase (CAT) werequantified by spectrophotometry. The results were analyzed by ANOVA andBonferroni´s test for multiple post hoc comparisons, considering significantdifferences at p