EFFECTS OF NARINGINE ON THE PROLIFERATION, MIGRATION AND INVASION OF COLON CANCER CELLS

MARCHIONATTI A; TOLOSA DE TALAMONI N; PAEZ MB; COLLIN A

Córdoba

Jornada; Jornada de Investigación Científica de la FCM de la UNC; 2018

s as a therapeutic tool has generated further research that recognize them, among others, for their antitumor properties. Naringin (NA), is a naringenin glycoside present in citric fruits, especially in grapefruit, which has various pharmacological and biological properties such as anti-inflammatory, antineoplastic, antioxidant, neuroprotective. OBJECTIVE: To know, at least in part, the molecular mechanisms by which NA has antineoplastic activity in cancer cells of colon cancer Caco-2 in culture. MATERIALS AND METHODS: Caco-2 cell line in culture were treated with NA or vehicle at different doses and exposure times. In them, several things were evaluated: 1- Cell proliferation by the technique of crystal violet or bromide of 3 (4,5 dimethyl thiazoyl-2) 2,5-diphenyltetrazolium, MTT. 2- Cell migration by the wound test. 3- Cell invasion by the zymography technique of metalloproteinases 2 and 9. 4- Differentiation by the measurement of alkaline phosphatase activity (AP). 5- Cellular redox state by quantification of the total glutathione content. The data were analyzed by ANOVA and the Bonferroni test as a post hoc test. RESULTS indicate that after treatment with NA, there was a decrease in cell proliferation, effect that was intensified with the increase of the dose and the time of exposure. Similarly, migration and cell invasion decreased in relation to the concentration of the flavonoid. The total content of GSH decreased and the activity of AP increased. In CONCLUSION, NA inhibits cell proliferation, migration and cell invasion and increases differentiation. These alterations are apparently mediated by the triggering of oxidative stress, probably from the depletion of GSH. Therefore, it is possible that NA could become a new tool for the treatment of human colon cancer.