Participation of hippocampus in the neuropeptide W regulation of food intake

SANTILLÁN ME; CARLINI VP; BIANCONI S; PORETTI MB; MEZA H; SCHIÖTH HB

Mar del Plata

Congreso; Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2018

SAIC

Neuropeptide W (NPW) is involved in the regulation of feeding behavior and energy homeostasis. The peptide and its receptor NPBWR1 are expressed in several regions of the brain, including the hypothalamus and hippocampus (Hi). Intracerebroventricular (ICV) administration of NPW can produce anorectic/orexigenic effect when is performed in the dark/light phase, respectively, and the hypothalamic injection stimulates food intake. The Hi is classically associated with learning and memory processes; however, recent studies implicate this structure in the regulation of food intake. Objective: to investigate the hippocampal role of NPW30 on feeding. Material and methods: male adult free-feeding rats were infused in the hippocampus with 0.5 μl per side, 1h after the onset of dark phase (feeding phase), with artificial cerebrospinal fluid (Control) and 24h later, with a single dose of NPW30 (0.3 or 3 nmol/μl), n≈13/dose. Food intake was registered 1, 4, 12 and 24 h after the infusions. Statistics: repeated-measures ANOVA and LSD post hoc test (significance: P