Effect of milk production on endometrial gene expression in grazing dairy cows

MADOZ LV,; JAUREGUIBERRY M,; DOMINGUEZ G,; RABAGLINO MB; PEREZ WALLACE S,; DE LA SOTA RL.; MIGLIORISI AL,; LORENTI N,

Eger

Congreso; XVIII Middle-European Buiatrics Congress & the XXVIII International Congress of the Hungarian Association for Buiatrics; 2018

Introduction: Cows with high milk production (HMP) have greater energy requirements andhigher feed consumption than medium milk production cows (MMP). This leads to a dramaticincrease in liver blood flow, which tend to elevate de metabolism of Estradiol (E2) andProgesterone (P4) affecting many aspects of normal reproductive physiology. The aim of thisstudy was to analyze the endometrial global gene expression in HMP and MMP on diestroususing RNA-seq technology.Methods: Grazing Holstein dairy cows (~50 DIM) without any clinical disease and BCS >2.50were included and allocated in two groups: HMP (~10.000kg/305d, n=3) and MMP(~7.500kg/305d, n=3). All cows were estrus synchronized with a Presynch-Ovsynch protocol.Blood was obtained on days 0, 4 and 9 to measure concentrations of P4 and E2 byquimioluminescence.Endometrial samples were collected using a biopsy instrument on day 9. The cDNA librariesfor RNA-seq were constructed using the TruSeq Stranded mRNA and sequenced using theHiSeq 1500 platform 2x150pb (Illumina Inc. CA, USA). Resulting sequence reads were alignedto the bovine reference genome (bosTau8), using the Rsubread package (R-software).Differentially expressed genes (DEG, p