Changes in the endometrial expression of growth factors associated with the estrus cycle in grazing dairy cows

MADOZ LV,; JAUREGUIBERRY M,; RABAGLINO MB,; QUINTANA S,; QUINTEROS L,; GIULIODORI M,; MIGLIORISI AL,; CHIOZZA LOGROÑO J,; DE LA SOTA RL.

Eger

Congreso; XVIII Middle-European Buiatrics Congress & the XXVIII International Congress of the Hungarian Association for Buiatrics; 2018

IntroductionThe aim of this study was to evaluate the effect of milk production level on estradiol (E2) and progesterone (P4) serum concentrations, and on endometrial expression of vascular endothelial growth factor (VEGF) and their receptors (VEGFR1 and VEGFR2), nodal growth differentiation factor (NODAL), epidermal growth factor receptor (EGFR), estradiol receptor (E2R) and progesterone receptor (P4R) on days 0, 4 and 9 of estrus cycle in grazing dairy cows.Materials and methodsHolstein cows (n=10) were randomly allocated in groups: high milk producer cows (HP, ~10.000 kg/305d), medium milk producer cows (MP, ~7.500 kg/305d) and anestrous cows. Blood and endometrium were sampled on days 0, 4 and 9 of estrus cycle. The mRNA expression was performed using real-time PCR with Beta-actin as housekeeping and samples of anestrus cows as calibrators. To analyze the relative level of expression, double- CT method was used. Data was analyzed with Proc GLIMMIX (SAS, 2016) by using a Gamma distribution and a log link function. Models included the fixed effect of time (0 vs. 4 vs. 9 d), treatment (HP vs. MP) and their interaction, and the random effect of the cow. Statistical significance was set at P