EZH2 OVEREXPRESION AND ITS EPIGENETIC MARK H3K27me3 IN PITUITARY TUMOR: ROL ON CELL CYCLE REGULATION

GUIDO CAROLINA; PETITI JUAN PABLO; SOSA LILIANA; MUKDSI JORGE; ZLOCOWSKI NATACHA; MOYANO GABRIELA; TORRES ALICIA

Buenos Aires

Congreso; Reunión Conjunta de las Sociedades de Biociencia 2017; 2017

Sociedades de Biociencia

Enhancer of zeste homolog 2 (EZH2) is a coreepigenetic regulator, which represses genes involved in tumorigenesis and playsa crucial role in cell cycle regulation. This protein catalyzes the repressivemark H3K27me3 and is associated with proliferation and worse outcome in severaltumor entities. Considering thefew epigenetics studies in pituitary tumors the aim of this study is to elucidatethe effect of EZH2 and H327me3 on cell cycle regulation in order to understandepigenetic´s tumor growth. For this propose we used experimental pituitarytumors induced by estradiol benzoate (30mg capsule) in adult Fisher male rats. Experiments were performedwith the adenohyphophysis and primary pituitary cell culture, from control andinduced-tumoral glands. Protein analysis of H3K27me3 and EZH2 was made throughWestern Blot and the mRNA levels of cell cycle regulators, cdkn1a, cdk4, ccdn1 and tp53 was quantified by qRT-PCR.  Statistical analysis: t-test (p.