A pathway driven approach suggests defective decidual NK cells in preeclampsia and endometrial disorders

CONRAD, KIRK P.; RABAGLINO, MARIA BELEN

San Diego

Encuentro; 65th Annual Scientific Meeting of the Society for Reproductive Investigation; 2018

Introduction: We recently reanalyzed our microarray dataset (GSE12767) basedon chorionic villous samples (CVS) obtained from women of ≈11.5 gestationalweeks who developed severe preeclampsia (PE-CVS) matched to CVS fromwomen with normal pregnancies. The results unexpectedly revealed a potentialrole for deficient (pre)decidualization (DEC) in the etiology of PE (Hypertension65:421-9, 2015). We further found that PE shared a partial molecular etiology withother endometrial disorders (ED), and the molecular pathology of the deliveredplacentas (DP) in PE (PE-DP) may reflect more consequence than cause of thedisease (Reprod Sci 24:91A, 2017; Placenta 4004:30289-8, 2017). However, tocomplete this work, pathway analysis was needed to reveal functionally-relatedgenes. The objective of this study was to employ a pathway-driven approach todiscover genes dysregulated in PE-CVS, ED and PE-DP.Methods: Publically available microarray datasets were reanalyzed with R todetermine differentially expressed genes (DEG, p