NARINGIN PREVENTS CHANGES IN THE MITOCHONDRIAL PHYSIOLOGY AND MORPHOLOGY OF KIDNEY IN THE EXPERIMENTAL DIABETES MELLITUS

PLAVNIK LM; PEREZ A; TOLOSA DE TALAMONI N; RIVOIRA M

Buenos Aires

Congreso; Reunión conjunta de la Sociedades de Biociencias; 2017

SAIC y otras sociedades científicas

We have previously demonstrated that the Diabetes mellitus (Dm.)inhibits the intestinal Ca²⁺ absorption, which was accompanied by oxidative stress. Other studies indiabetic rats have shown enhanced glomerular filtration rate with raisedurinary output and reduced Ca2+ reabsorption. Alteration of the redox state wasobserved in kidney mitochondria, with reduced ATP synthesis, changes in calciumhomeostasis and increased biogenesis. The objective of this study was todetermine if naringin (NAR), a natural antioxidant, could block the alterationsin the renal morphology, mitochondrial redox state and energy metabolism fromkidney in diabetic rats. Adult male Wistar rats were divided in: 1) controls,2) streptozotocin (STZ) treated (diabetic rats) and 3) STZ + NAR treated.Mitochondria were isolated from renal tissue from each group of animals by differentialcentrifugation. In the mitochondrial extracts the activities of the enzymesisocitrate dehydrogenase (ICDH-NAD), malate dehydrogenase (MDH-NAD), superoxidedismutase (SOD), catalase (CAT), glutathione (GSH) and superoxide anion levels(˙O2-) were determined by spectrophotometry. Kidney morphology was analyzed byhistological studies. Results were assessed by one-way ANOVA and Bonferronimultiple comparison test. The STZ treatment decreased the enzymatic activitiesof ICDH and MDH and the GSH content, which were avoided by NAR. STZ increasedthe SOD and CAT activities and the contents of ˙O2- and protein carbonyl. Allthese effects were blocked by NAR, except CAT activity. In addition, diabeticrats showed disorganized glomeruli, which were smaller than those fromcontrols. The epithelial cells from distal convoluted tubules were alsodecreased by STZ. All these morphological changes were avoided by NAR. Inconclusion, NAR abrogates morphological alterations in the diabetic kidney and changesin the energy metabolism from the renal mitochondria improving the redox stateof these organelles.