Bronchiolar epithelium participation in the LPS-neonatal prevention of asthma

LUCIANA N GARCÍA; MARÍA VICTORIA SCALERANDI; ELISA M URIBE ECHEVARRÍA; CAROLINA LEIMGRUBER; JUAN PABLO NICOLA; AMADO A QUINTAR; CRISTINA A MALDONADO

Buenos Aires

Congreso; IV LASID Meeting, LXIII Argentinean Society for Immunology Meeting, II French-Argentinean Immunology Meeting; 2015

Sociedad Argentina de Immunología, French- Argentinean Immunology Congress

Club cells (CC) are clue in the bronchiolar epithelium (BE) homeostasis and express Host Defense proteins (HDp) like the antinflammatory: CCSP, the lectin SP-D that can exert antiallergic effects. The allergic asthma inflammation (AAI) induces mucous CC metaplasia and decreases their HDp contain. Recent studies suggest that microbial stimulus (MS) in early-life may protect from asthma developing. Accordingly, our aim was to analyze CC response to AAI developed in mice that were neonatally exposed to E. coli LPS intranasally (in.). Balb/c mice were intranasally (in.) pre-exposed to 5μl of PBS or LPS (200mg/ml), between days 3-13 after delivery (AD). 4 and 6 weeks AD mice were intraperitoneal sensitized by 100μl of ovoalbumin(OVA)/ Alumm (1mg/1mg/ml) injection and assigned into groups (n=10). Then, they were in. challenged with OVA (1mg/ml) (LPS/OVA and PBS/OVA group) or vehicle (LPS and PBS group) for 10 days. 24hs later, lung and bronchoalveolar lavage (BAL) were obtained. CC of LPS/OVA mice showed, versus OVA, fewer PAS-positive reaction (p