Glycerol-3-phosphate dehydrogenase isoforms expression in flight muscles of Triatoma infestans.

MARÍA M. STROPPA; MARIANA E. LAGUNAS; CARLOTA S. CARRIAZO; BEATRIZ A. GARCÍA; GREGORIO IRAOLA; YANINA PANZERA; NELIA M. GEREZ DE BURGOS

Rosario

Congreso; XV Congreso Latinoamericano de Genética, XLI Congreso Argentino de Genética, XLV Congreso de la Sociedad de Genética de Chile, II Reunión Regional SAG-Litoral; 2012

Asociación Latinoamericana de Genética (ALAG)

In Triatoma infestans (T. infestans) flight muscles, glycerol-3-phosphate dehydrogenase (GPDH) isoforms are differentially expressed during development and between sexes. GPDH1 is involved in flight metabolism and GPDH2 provides lipid precursors. We studied isoforms expression in flight muscles of natural populations and laboratory colonies of T. infestans, and analyzed intake and temperature effects in transcript patterns in laboratory colonies. We determined GPDH total activity and performed semiquantitative RT-PCR and non-denaturing PAGE revealed with specific activity. Total activity was lower in first and second laboratory generations than in natural populations. We observed concordance among RNA level and isoform specific activity. We demonstrated that GPDH1 predominates in adult T. infestans flight muscle from natural population and laboratory first and second generations. The GPDH2 expression of natural population compared with the first and second laboratory generations increased and GPDH1 decreased. Under laboratory conditions, the increase of the intake time from 30 to 120 min. promoted transcript patterns changes: before last molt, GPDH1 and GPDH2 increased 2 and 25 fold, respectively; in young adults, GPDH1 decreased 20% and GPDH2 increased 40%; in 30 days old adults, the GPDH2 was 20% higher and GPDH1 had no significant modification. Patterns differed at 22ºC and 28°C temperatures. At 22°C pattern had delayed changes. Results showed isoforms adaptive expression in flight muscles, possibly due to alternative splicing and consistent with metabolic requirements.