ATPases activities in gills of crab Neohelice granulata from contrasting habitats of Mar Chiquita coastal lagoon: differential response to hyporegulation

GONZÁLES, SM, PINONI, SA, LÓPEZ MAÑANES AA

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2012 vol. 32 p. 63 - 63

0327-9545

Differential adjustments in key biochemical parameters (i.e. enzymes involved in osmoregulatory responses in euryhaline crabs) could be related to successful occupancy of contrasting habitats within a same area. The aim of this work was to determine ATPase activities in anterior (1-5) (AG) and posterior (6-8) (PG) gills in male crabs from mudflat (M) and saltmarsh (SM) exposed for 10 days to 35 (osmoconformation) and 45‰ salinity (S) (hyporegulation). 2500xg supernatants were used as enzyme extract (homogenization medium: 0.25M sucrose/0.5mM EGTA-Tris pH7.4). ATPase activity (nmol Pixmin-1xmg prot-1) was assayed by measuring 5mM ATP hydrolysis in (mM): 20 imidazole pH7.4/130 NaCl/0.5 EGTA/ 1 ouabain/1 NaN3 without (Basal ATPase activity: BA) or with bafilomicin A1 (1μM). The difference of both assays represents VH+ ATPase activity (VHA). t-test was used as statistical analysis (p