IIMYC   23581
INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Unidad Ejecutora - UE
artículos
Título:
PROTEOLYTIC ACTIVITY IN HEPATOPANCREAS OF THE EURYHALINE CRAB Neohelice granulata: RESPONSE TO HYPEREGULATION AND FEEDING
Autor/es:
MÉNDEZ, E; LÓPEZ MAÑANES, A; PINONI, S
Revista:
BIOCELL
Editorial:
INST HISTOL EMBRIOL-CONICET
Referencias:
Lugar: Mendoza; Año: 2012 vol. 3 p. 61 - 61
ISSN:
0327-9545
Resumen:
Hyperegulation is a physiological status with enhanced energy demands.
Biochemical adjustments in extrabranchial organs (i.e.
modulation of digestive enzymes in hepatopancreas) could occur
in response to hyperegulation and could be affected by feeding status.
However, integrative studies on these adjustments are lacking.
The aim of this work was to determine proteolytic activity (Prot) at
different times after feeding in hepatopancreas of N. granulata from
Mar Chiquita coastal lagoon acclimated to different salinities (S)
(35S:osmoconformation; 10S:hyperegulation). Adult male
crabs exposed for 10 days to 35 and 10S (feeding regime: 3 times
a week), were unfed for 5 days (preingesta=PI) and then individually
fed. Prot was determined in PI, immediately (t0) and at 1, 8,
24, 48, 72, 120 h post-ingesta. Prot (enzyme units×h-1×mg protein-
1) was assayed by measuring azocasein (1%p/v) hydrolysis in
Tris-HCl, 100 mM, pH 7.5. PI and t0 values were similar both in
35 and 10S. In 35 S, Prot was higher between 8 (8.23 ± 0.2)
and 72 h (8.9±1) post-ingesta than in PI (5.04 ± 0.95). In 10S, no
differences occur between PI (4.2±0.87) and any time post-ingesta.
No differences were found between osmoconformation and
hyperegulation conditions in either PI or at any time post-ingesta
(p=0.001). The results shows a different Prot responses to feeding
in 35 and 10S suggesting a relationship between post-feeding
adjustments and osmoregulatory status.