IMPACT OF TWO BREAST-MILK DERIVED POTENTIAL PROBIOTICS ON THE GUT MICROBIOTA OF AN OBESE CHILD

REIHEIMMER J; SIVIERI K; ODDI S; BURNS P; HUBER P; VINDEROLA G

Conferencia; Gut Microbiota for Health World Summit; 2019

American Gastroenterological Association

ABSTRACTObjectives. The gut microbiota is emerging as a new factor in the development of obesity. Thisstudy aimed at evaluating the effects of two potential probiotic strains isolated from humanbreast milk, Lactobacillus plantarum 73A and Bifidobacteria animalis subsp. lactis INL1 on themicrobiota of an obese child using the Simulator of the Human Intestinal Microbial Ecosystem(SHIME®), a dynamic model of the human gut.Methods. The SHIME was run on the following program: stabilization (2 weeks), L. plantarum73A (2 weeks, 1010 CFU/ day), washout (1 week) and L. plantarum 73A/B. lactis INL1 (1010CFU/ day each, for 2 weeks). Analysis of the intestinal microbial composition (16S rRNA geneamplicon sequencing) was performed, short-chain fatty acids (SCFAs) levels were measured bygas chromatograph and ammonium (NH4+) concentration was assessed by an ammoniaselective ion electrode.Results. Both treatments significantly decreased ammonia concentration: from 483 ppm(stabilization) to 434 ppm (L. plantarum 73A, p = 0.0001), and from 530 ppm (washout) to 460ppm (mix of strains, p = 0.015). No changes in SCFA were observed. Metagenome analysis ofintestinal microbiota showed that both strains influenced the increase on phyla Bacteroidetesmodifying the microbiota structure (p= 0.04). In addition, some Clostridium species decreasedwhereas Roseburia intestinalis significantly increased, beneficial specie linked to a healthy gut.Conclusion. Breast milk-derived L. plantarum 73A and B. animalis subsp. lactis INL1 have apotential to undergo further studies to promote gut health in obese child.