Whole genome analysis of the antibiotic resistant determinants and mobile elements present in the uncommon pathogens Pseudomonas chlororaphis and Shewanella spp.

TERESE LAZZARO; SYLVIA UONG; SABRINA MONTAÑA; GISELA PARMECIANO DI NOTO; MARISA ALMUZARA; CECILIA QUIROGA; ANDRES IRIARTE; CARLOS VAY; MARÍA SOLEDAD RAMÍREZ

Congreso; ASM Microbe 2017; 2017

American Society for Microbiology

Background: Implementation of new technologies in the diagnostic laboratorysetting have contributed to the increase in identification of uncommon pathogens.These pathogens are often environmental microorganism with high level ofresistance to several antibiotics transforming them into potential reservoirs ofantibiotic resistance traits. Horizontal genetic transfer is a key mechanism in thedevelopment of antibiotic resistance.Methods: The draft genome sequence of Pc190 and Shew256 were obtained withIllumina MiSeq-I and Nextera XT DNA library. De novo assembly was performedwith SPADES assembler 3.1.0 version. RAST was used to predict the open readingframes (ORF) and the predictions were confirmed using BLAST (version 2.0).Further genomic analysis was carried out.Results: The draft genomes of Pc190 and Shew256 consist of 7,453,526-bp and5,365,006-bp, respectively. By RAST server 6,986 and 4,766 protein-coding geneswere predicted within Pc190 and Shew256, respectively. The corresponding G+Ccontents were 63.0% and 47,9% for Pc190 and Shew256, respectively.Twelve resistant genes coding for resistance to seven different antibiotics familieswere present in Shew256, including β-lactamases such as PER-2. Several mobileelements were observed, such as insertion sequences, transposon, phages and thepresence of an unusual class 1 integron. In Pc190 genome, resistant gene werefound including β-lactamases and Metallo-β-lactamases, several efflux pumps,phages were also found.Conclusion: A variety of resistance genes, which can explain the multidrugresistance phenotypes observed in both strains, as well as the presence of mobileelements, that can serve as tools in the mobilization of these determinants, wereobserved. We also highlight the importance of the isolation of uncommon multidrug resistance pathogens from clinical specimens