Design of derivatization strategies for anticonvulsants determination using molecular fluorescence

GUIZZADO VIRGINIA; FERNÁNDEZ LUCIANA; PIRCHIO PAULA; PERALTA CECILIA MARIANA; FERNÁNDEZ LILIANA PATRICIA; ACOSTA MARÍA GIMENA

Congreso; VI Reunión Internacional de Ciencias Farmacéuticas; 2021

Abstract: Emerging pollutants (EC) are chemical substances that reach the environment as a result of different human activities and generate problems in the metabolism of living beings and in human health in particular. Anticonvulsants are drugs, which have been considered as EC causing interest in recent years. Within this group are: Phenytoin (FI), Phenobarbital (FB), Topiramate (TOP).The main objective of this work is to develop different derivatization strategies that allow the determination of these three EC employing fluorescence detection, reaching detection limits compatible with the concentrations found in environmental samples.The derivatization of these CEs consists of reactions with appropriate agents and UV radiation in order to obtain products that are easily detectable spectrofluorimetrically. Several factors that affect the derivatization reaction were studied, such as nature and concentration of the fluorophore, concentration of ligand, pH, effect of different surfactants, etc. Promising results have been obtained using rhodamine B solution (1x10-9 mol L-1) as fluorophore agent. Thus, the experimental conditions were: λex = 555 and λem = 575; slits width 5-5.The developed strategies will be evaluated into schemes that combine high-resolution liquid chromatography associated with fluorescent detection with the aim of automate the methodology and allowing the simultaneous determination of the CEs.