On-Line Photoinduce Fluorescence for Carbamazepine Determination after Multivariate Optimization

CECILIA HENESTROSA; CECILIA M. PERALTA; LESLIE ARAGÓN; LILIANA P. FERNÁNDEZ; GIMENA ACOSTA

American Journal of Analytical Chemistry

Scientific Research Publishing

Año: 2019 vol. 10 p. 513 - 528

2156-8251

This work presents a simple and sensitive flow injection fluorimetric methodfor the determination of carbamazepine (CBZ) in pharmaceutical samples. Itis based on the fluorescence signal measurement of the product generated byphotoinduced (λex = 251 nm; λem = 434 nm) using a flow injection manifoldcontaining an on-line homemade photoreactor. In order to optimize the experimental setup, the variable conditions were studied using a multivariateoptimization method, thus finding the set of optimum parameters accordingto the evaluated responses (sensitivity and sampling frequency) and minimizingthe number of experiments performed. Under optimal experimental conditions, linear relationship with good correlation coefficient (0.9956) was found between the fluorescence intensity and CBZ concentration in the range of 0.13 - 40 μg∙mL−1. The limit of detection was 0.04 μg∙mL−1. The precision of the method was satisfactory; the values of relative standard deviations were better than 4.36%. The proposed method was validated and successfully applied to the determination of CBZ in pharmaceuticals with good accuracy; being the recovery values from 89.45% - 112.20%.