INVESTIGADORES
PERALTA cecilia mariana
artículos
Título:
O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI)-spectrofluorimetry
Autor/es:
CECILIA M. PERALTA; LILIANA P. FERNÁNDEZ
Revista:
Journal of Pharmaceutical Analysis
Editorial:
Elsevier
Referencias:
Año: 2014 vol. 4 p. 368 - 373
ISSN:
2095-1779
Resumen:
A simple, eco friendly, sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-(β-hydroxyethyl)rutosides. The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-(β- hydroxyethyl)rutosides fluorescence enhancement, increasing considerably the sensitivity of detection. All the variables affecting the fluorescence intensity were studied and optimized. The flow rate was 5 mL/min with detection at 450 nm (after excitation at 346 nm). A linear correlation between drug amount and peak area was established for O-(β-hydroxyethyl)rutosides in the range of 0.01 - 200 μg/mL with a detection limit of 0.001 μg/mL (s/n = 3). Validation processes were performed by recovering studies with satisfactory results. The new methodology can be employed for the routine analysis of O-(β-hydroxyethyl)rutosides in bulks as well as in commercial formulations.
