Quantitative proteomic and transcriptional analyses reveal degradation pathway of gamma- hexachlorocyclohexane and the metabolic context in the actinobacterium Streptomyces sp. M7

SINELI, P.E.; HERRERA, H.M ; CUOZZO, S.A.; DAVILA COSTA, J.

CHEMOSPHERE

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2018

0045-6535

Highly γ-hexachlorocyclohexane (lindane) contaminated areas were reported worldwide. Low aqueous solubility and high hydrophobicity make lindane particularly resistant to microbial degradation. Physiological and genetic Streptomyces features make this genus more appropriate for bioremediation compared with others. Complete degradation of lindane was only proposed in the genus Sphingobium although the metabolic context of the degradation was not considered. Streptomyces sp.M7 has demonstrated the ability to remove lindane from culture media and soils. In this study, we used MS-based label-free quantitative proteomics, RT-qPCR, and exhaustive bioinformatic analysis to understand lindane degradation and its metabolic context in Streptomyces sp. M7. We identified the proteins involved in the up-stream degradation pathway. In addition, results demonstrated that the mineralization of lindane is feasible since proteins from an unusual down-stream degradation pathway were also identified. Degradative steps were supported by active catabolism that supplied energy and reducing equivalents in the form of NADPH. To our knowledge, this is the first study in which degradation steps of an organochlorine compound and metabolic context are elucidated in a biotechnological genus as Streptomyces. These results serve as a basement to study other degradative actinobacteria and to improve the degradationprocesses of Streptomyces sp. M7.