?ROLE OF THE L10 RIBOSOMAL PROTEIN IN THE UV-B RESPONSE IN PLANTS?

FALCONE L.; CASATI P.

Mar del plata

Congreso; XLIII Reunión Annual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB).; 2007

ROLE OF THE L10 RIBOSOMAL PROTEIN IN THE UV-B RESPONSE IN PLANTS Falcone Ferreyra, Lorena; Casati, Paula. CEFOBI. Fac. Cs. Bioq. y Farm. UNR. Suipacha 531. 2000.Rosario. Argentina. e-mail:mfalcone@fbioyf.unr.edu.ar Plant development and physiology are affected by UV-B radiation. In maize, a number of genes encoding ribosomal proteins are regulated by UV-B, one example is L10. In A. thaliana, three genes encode putative L10s (rpl10 A-C). We measured mRNA levels by real time RT-PCR: C mRNAs are increased, B is decreased and A is not changed by UV-B. In order to investigate the role of each RPL10 protein in UV-B responses, A. thaliana T-DNA mutant plants in the rpl10 genes were analyzed. In rpl10C mutants, RPL10A mRNAs are strongly increased in UV-B treated plants. Heterozygote rpl10A mutant plants expressing 5-fold lower transcript levels than WT showed a similar pattern of rpl10B and C expression compared to WT under UV-B, but surprisingly, rpl10A expression is induced. We further assayed the expression of marker genes regulated by UV-B (CHS, HY5,UGT): there are no significant differences between rpl10C and WT plants, but in rpl10A deficient plants, the induction of these genes by UV-B is reduced. These data suggest a possible involvement of RPL10A in regulation of gene expression as it has been described in other organisms, this role is under investigation.