MOLECULAR TYPING USING RAPD-PCR IN GROUP B STREPTOCOCCAL ISOLATED FROM NEWBORNS IN MISIONES.

LACZESKI, ME; NOVOSAK, MG; BOBADILLA, FJ; ALVARENGA, A; VERGARA, MI

Mar del Plata

Congreso; SAIB 2015. 51 Annual Meeting; 2015

Argentine Society for Biochemistry and Molecular Biology

Streptococcus agalactiae, Group B Streptococcus(GBS) is considered to be the major cause of neonatal sepsis and meningitis ofbacterial origin. The Random Amplified Polymorphic DNA analysis (RAPD) is an accessible and sensitive method based on the use ofarbitrary primers to amplify polymorphic segments of DNA and a powerful methodto differentiate GBS strains. Theaim of this study was to determine if their clonal relationshipbetween the strains of group GBS isolated from newborns in the province of Misiones, Argentina. For this purpose, eleven strains of GBSinvasive isolated from blood cultures and cerebrospinal fluid (CSF) werestudied by RAPD using threeprimers, designated OPS11, OPB17, and OPB18. The PCR mixture consisted of buffer(10 mM Tris-HCl [pH 8.3], 50 mM KCl; 2.5 mM MgCl2), 100 mM each of the fourdeoxynucleoside triphosphates (INBIO, Argentina), 0.4 mM primer and 2.5 U of Taq DNA polymerase (INBIO, Argentina) ina total volume of 25 ml. Eleven different band patternswere obtained by RAPD-PCR with significantbands ranging from 100-5000  bp. In conclusion, the RAPD-PCR assayshowed that each of the isolated clones belonged to a different RAPD genotype, revealing that neonatalinvasive GBS infections were not epidemiologically related. Thegenetic diversity studies provideinsight into the regional epidemiologyof this disease and adapt prevention strategies.