MOLECULAR PROFILES OF VIRULENCE OF Streptococcus agalactiae IN MISIONES, ARGENTINA

LACZESKI M

Londres

Congreso; 7th World Congress on Healthcare & Technologies; 2016

Hosting Organization: Conference Series LLC

              Streptococcus agalactiae (GBS) is the leading cause of severeinvasive infections in infants less than three months. Meningitis, pneumoniaand sepsis are the leading cadres in these children. Infection is primarilyacquired vertically from mothers colonized with GBS. GBS has many virulence factors, the capsular polysaccharide isone of the most important and are associated with different surface proteins ofantigenic characteristics, such as α, β, Rib, HylB, Lmb, C5a peptidase, FbsA,FbsB and CylB which are encoded by several genes associated with virulence andhost interaction acting on bacteria involved in invasiveness. The study ofsurface protein antigens is important for the understanding of the pathogenesisand epidemiology of infection and several these antigens have been proposed ascomponents of multivalent conjugate vaccines. This study was carried out inorder to study the molecular profiles of virulence in GBS strains. Two hundred isolates of vaginal swabs from pregnantwomen from Misiones (Argentina), were studied. Virulence genes those encoding:C protein (bac and bca), Rib protein (rib), laminin (lmb),hyaluronidase (hylB), c5a-peptidase (scpB), FbsA and FbsB protein (fbsA and fbsB) and β-hemolysin (cylB),were investigated by conventional PCR. Virulence genes were simultaneously identifiedin 73 isolates (36.5%). fbsA, fbsB and cspBgenes were detected in 100% of the strains. Other genes studied were detected in thesefrequencies: cylB(95%), lmb (94%), bca (87,5%), rib (85,3%), hylB (81%) and bac (58%). The high frequency of detection of fbsA,  fbsB and cspBgenes, suggesting assessment of their inclusion in future vaccines.