Producing high value aroma compounds by whole-cell biocatalysis using Aspergillus niger LBM055

VELÁZQUEZ, JUAN; SADAÑOSKI, MARCELA; ZAPATA, PEDRO; COMELLI, NORA; VILLALBA, LAURA

Arabian Journal for Science and Engineering

Springer

Año: 2023

2193-567X

The aims of this article were to optimize and assay the production of high value aroma compounds at a bench-scaleby the bioconversión capacity of Aspergillus niger LBM055 strain using R-limonene as platform chemical. Temperature, pH and agitation effecton R-limonene bioconversión were evaluated. Optimal R-limonene bioconversión wasmet at 30.5 ◦C, 100 rpm and pH 6.2, mean while be styield of high molecular weights products was obtained at 28◦C, 100 rpm and pH 5.4. Conditions of be styield of high molecular weights were selected to apply the matabench-scale biorreactor α-pinene, βpinene, citral, linalool, carvone, carveol and myrcene were identified. Also, a mixture of carvone, carveol and limonene oxide at a high concentration of 3.1 g/L, was identified by GC-MS analysis. Optimization análisis demonstrated that with the same substrate and fungal strain, it was posible to obtaind ifferent bioaromas compounds only by changing the operating conditions.