RENAL CARCINOMA OF CLEAR CELLS: THE ROLE OF PROTEIN BMP7 AND ITS DIFFERENTIAL EXPRESSION

OLEA GABRIELA; MELANA COLAVITA JUAN PABLO; BARNES TAMARA; PALOMAR LUCAS; LOMBARDO DANIEL; AGUIRRE MA. VICTORIA

BUENOS AIRES

Jornada; Reunión conjunta de sociedades de biociencias; 2018

The complex biology of the ccRCC, added to its unpredictable clinical behavior, highlights the need to determine other biomarkers to evaluate neoplastic progression, as well as to generate new therapeutic alternatives taking into accounts the heterogeneity intratumoral.The objective of the following work is to determine the differential expression of BMP-7 of ccRCC, in stem cells of embryonic phenotype. In order to correlate their expression with clinical-pathological parameters for their potential use as predictors of oncological progression and / or therapeutic targets. For them, tumor samples were collected from patients diagnosed with CRC with radical or partial nephrectomy from the Urology Department of the J.R. Hospital. Vidal from the city of Corrientes. Clinical-pathological evaluation: performed at the Pathological Anatomy Service of the J.R. Hospital. Vidal. Sections of 4 μm of CCR tumors and normal adjacent kidney tissue were included in paraffin and placed on silanized slides. The conditions for antigenic and incubation unmasking for the BMP-7 (1:200) protein were adjusted. The analysis of the expression of BMP7 in renal carcinoma revealed that this protein is expressed differentially between the distal (control), core and periphery regions at the perinucleolar level and in turn in relation to the type of invasion presented by the carcinoma. While expression of BMP7 was lower in the distal region. This investigation revealed that, compared to the distal region taken as a control, BMP7 expression was positively regulated according to the degree of invasiveness, and even the expression of said protein in tumors. With metastasis was nuclear. The increase in the expression of BMP7 in cells positively marked for said protein in the invasive stage of the disease was evidenced. Future analyzes will focus on the expression by molecular biology of said biomarker and the comparison with other potential biomarkers such as the case of the plutipotentiality protein OCT4.