ANALYSIS OF DEATH BY APOPTOSIS IN EMBRYONIC AND NEONATAL OVARIES OF Columba livia (Aves: COLUMBIFORMES)

OLEA GABRIELA; AGUIRRE MA. VICTORIA; LOMBARDO DANIEL

Chascumús, Buenos Aires.

Jornada; XVJornadas Anuales de la Sociedad Argentina de Biología; 2013

Sociedad Argentina de Biología

Programmed Cell Death (MCP) is a common step in the germ line during development. The knowledge of these events is essential for the interpretation of processes involved in the MCP of gametes. However, in birds embryonic and neonatal level, there are only references to model species as Gallus gallus domesticus and Coturnix coturnix. The aim of this study was to examine the MCP process linked to the differentiation of oogonia in embryos and hatchlings of Columba livia. Analysis was performed on the expression of Bcl family proteins (Bax, Bcl2). For detecting Bcl2 activity, was used as the primary antibody rabbit polyclonal anti Bcl2 anti mouse origin, and to Bax, the primary antibody anti Bax monoclonal mouse anti-human origin, rat and mouse. Apoptosis was evident in the functional ovary both in marrow and in oocytes, increasing post-hatching stage. These results highlight the death of bone marrow cells and ovarian oocytes during the embryonic stage and post-natal. They serve as a basis for future studies related to the quantification from apoptotic indices at each stage of development.