EXPRESSION RECEPTOR GnRH (GnRH) ON CELLS THE GERM LINE COLUMBA LIVIA (BIRDS: COLUMBIFORMES)

OLEA GABRIELA; AGUIRRE MA. VICTORIA; LOMBARDO DANIEL

BUENOS AIRES

Jornada; VI Jornadas Internacionales de Instituto de Reproducción Animal (INITRA); 2014

UNIVERSIDAD DE BUENOS AIRES-FACULTAD DE CIENCIAS VETERINARIAS

There is strong evidence of the presence of gonadotropin releasing hormone (GnRH) and its receptor (GnRHR) in the gonads of various vertebrate species. The expression of GnRH and its receptor in the sexual organs has been demonstrated in fish, amphibians, birds and mammals. From these autocrine and paracrine neuropeptide signaling regulate development and gonadal function, including steroidogenesis, spermatogenesis and folliculogenesis. Knowledge of these events gonadal endocrine regulation during development of birds is rare and has only been described embryonic processes steroidogenesis in Gallus gallus domesticus and Coturnix coturnix. Therefore the aim of this work is to characterize the GnRH receptor expression in embryonic testes and ovaries and post-natal Columba livia to provide information about the process of steroidogenesis in birds. For this revealed in histological preparations testis and ovary expression GnRH in corresponding to embryos stages 41, 43 and neonates 2, 5, 7 and 75 days post-hatch, using an anti GnRHR PABX (1 mg / ml) at a working dilution of 1: 800 incubated for 60 min. at 37; and revealed using the indirect protocol "L-streptavidin biotin". Samples of blood corresponding to these stages were obtained prior to euthanasia and immediately centrifuged at 1500 RPM for 5 minutes to isolate the serum, which was kept in freezer - 4 ° C until use. From the results it was observed that in stadiums analyzed, GnRH receptor revealed immunodetection the presence of specific labeling in the plasma membrane and in the peri nuclear area of the the germline cells of ovary in stages pre -eclosión and testicles juveniles. Future analysis will focus on correlating these results with the hormone levels in plasma from techniques Radioimmunoassay (RIA).