Protective effects of dexmedetomidine in early experimental sepsis

RONCHI JULIETA; OLEA GABRIELA; AGUIRRE MA. VICTORIA; STOYANOFF TANIA

Buenos Aires

Jornada; IX Jornadas de Jóvenes Investigadores; 2019

FCVet-UBA

Sepsis and septic shock are characterized bylife-threatening organ dysfunction caused by adysregulated host response to infection. Theyare common and fatal disorders, representingthe major determinant of morbidity andmortality in intensive care units (ICU). Thishighlights the importance of the study ofnew therapeutic strategies to mitigate theseeffects. Dexmedetomidine (DEX) is a commonsedative drug used in the intensive care unit andseveral evidences have shown that may inducea variety of cytoprotective effects on earlysepsis. In this study, we aimed to evaluate theprotective effect of DEX on a murine model oflipopolysaccharide (LPS) induced-endotoxemiaby histological and biochemical examinations.Male inbred Balb/c mice were divided into fourexperimental groups: I) Control; II ) LPS ( 8mg / kg , ip) , III ) DEX ( 50µg/ kg , ip ) andIV ) DEX+LPS. Histopathological analysesfor renal, pulmonary and hepatic tissues, andbiochemical determinations were performed at24h post LPS administration. DEX+LPS groupshowed a significant improvement in renalfunction, as well as, a significant attenuationof histopathological alterations induced byLPS from 24 hs. Our data revealed that DEXtreatment remarkably improving metabolicacidosis and hypoglycemia. This studydemonstrates that DEX exerts protective effectson kidneys, liver and lungs in endotoxemicmice. Further in vivo studies will be performedto determine the molecular mechanismsinvolved in these improvements.