INVESTIGADORES
NOUHRA Eduardo Ramon
artículos
Título:
Morphological and molecular characterization of selected Ramaria mycorrhizae
Autor/es:
NOUHRA E.; HORTON T.; CAZARES E.; CASTELLANO M.
Revista:
MYCORRHIZA
Editorial:
Springer Verlag
Referencias:
Año: 2005 vol. 15 p. 55 - 59
ISSN:
0940-6360
Resumen:
Ramaria species are conspicuous mycorrhizal symbionts of conifers in the Pacific Northwest. Here we collected and identified sporocarps and associated ectomycorrhizae of Ramaria acrisiccescens Marr & Stuntz, R. cyaneigranosa Marr & Stuntz, R. sandracina Marr & Stuntz, R. celerivirescens Marr & Stuntz, and R. flavobrunnescens var. aromatica Marr & Stuntz. An internal transcribed spacer (ITS)- restriction fragment length polymorphism pattern was observed for each of the Ramaria species and used as a diagnostic tool to support the identification of mycorrhizae occurring in mats below the sporocarps. We provide a description of the ectomycorrhizae of Ramaria, which exhibit similar macro- and microscopic characteristics such as ramification pattern, coloration, abundance of  mycelial strands and emanating hyphae, mantle morphology and chemical reactions of mantle and mycelial strands with KOH, Fe SO4  and Melzer´s reagent. Sequences of the ITS region for each of the species are deposited in the Gen Bank.